| 货号: S218311 |
| 产品全名: UBE2E3 兔多抗 |
| 基因符号 UBCH9; UbcM2 |
| UNIPROT ID: Q969T4 (Gene Accession – BC003554 ) |
| 背景: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. |
| 抗原: Fusion protein of human UBE2E3 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 30-150;WB: 200-1000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling, Cell Biology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 218311(UBE2E3 Antibody) at a dilution of 1/40(Nucleus and Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the fusion protein and then with 218311(Anti-UBE2E3 Antibody) at dilution 1/40. | 
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 218311(Anti-UBE2E3 Antibody) at a dilution of 1/40. | 
In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with fusion protein and then with D224137(Anti-UBE2E3 Antibody) at dilution 1/40. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: Mouse kidney tissue lysate;
Primary antibody: 218311(UBE2E3 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 minutes | |
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