| 货号: N263595 |
| 产品全名: SUMO Conjugating Enzyme UBC9 兔单克隆抗体 |
| 基因符号 UBE2I; UBC9; UBCE9; SUMO-conjugating enzyme UBC9; SUMO-protein ligase; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; Ubiquitin-conjugating enzyme E2 I; Ubiquitin-protein ligase I; p18 |
| UNIPROT ID: P63279 |
| 背景: The process of SUMO-1 conjugation is similar to that seen with ubiquitin and other forms of post-translational protein modification. Like ubiquitin, SUMO-1 is conjugated to its target protein by the coordinated action of ubiquitin conjugation enzymes E1, E2 and E3. Ubc9 (or ube2M) is a highly conserved, 158 amino acid protein that acts as a SUMO-1 conjugating enzyme. Ubc9 binds to target proteins through their SUMO-1-CS (consensus sequence) domains and interacts with SUMO via the structurally conserved amino-terminal domain. |
| 抗原: A synthetic peptide of human UBE2I/UBC9 |
| 经过测试的应用: WB,IHC-F,IHC-P,ICC/IF,IP |
| 推荐稀释比: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Monoclonal |
| 克隆编号: R01-6I6 |
| 分子量: Calculated MW: 18 kDa; Observed MW: 18 kDa |
| 亚型: IgG |
| 纯化: Affinity Purified |
| 种属反应性: Human, Mouse and Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
| 研究领域: Epigenetics&Histone modifications |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Western blot analysis of UBE2I/UBC9 in Hela, A549, HL-60 lysates using UBE2I/UBC9 antibody | 
Immunocytochemistry analysis of SUMO Conjugating Enzyme UBC9 (green) in Hela using SUMO Conjugating Enzyme UBC9 antibody,and DAPI(blue). | 
Western blot analysis of UBE2I/UBC9 in 3T3, PC-12, MEF lysates using UBE2I/UBC9 antibody. | 
Immunohistochemistry analysis of paraffin-embedded Human tonsil using UBE2I/UBC9 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. |
|
