武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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SPP1 Rabbit pAb

Anti-SPP1 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S210499

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S210499
产品全名:   SPP1 兔多抗
基因符号   OPN; BNSP; BSPI; ETA-1
UNIPROT ID:   P10451 (Gene Accession – BC007016 )
背景:   The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.
抗原:   Fusion protein of human SPP1
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 25-100;WB: 500-2000;ELISA: 1000-2000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Cardiovascular, Signal Transduction, Stem Cells, Cancer
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 210499(SPP1 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the fusion protein and then with 210499(Anti-SPP1 Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: 293T, HepG2, U251 cell lysates;
Primary antibody: 210499(SPP1 Antibody) at dilution 1/600;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds
           引用文献