武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

邮箱:2716620741@qq.com

咨询:027 – 63497286 

Raf1 Rabbit pAb

Anti-Raf1 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:N225480

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   N225480
产品全名:   Raf1 兔多抗
基因符号   RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1
UNIPROT ID:   P04049
背景:   Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2.
抗原:   The antiserum was produced against synthesized peptide derived from human C-RAF. AA range:251-300
经过测试的应用:   ICC/IF,WB,IHC-F,IHC-P,ELISA
推荐稀释比:   WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
分子量:   Calculated MW: 73 kDa; Observed MW: 73 kDa
亚型:   IgG
纯化:   Affinity Purified
种属反应性:   Human, Mouse and Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
研究领域:   Signal Transduction
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded rat kidney tissue using Raf1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of Raf1 in HepG2 lysates using Raf1 antibody.

Western blot analysis of Raf1 in rat liver lysates using Raf1 antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of Raf1 in mouse liver using Raf1 antibody(red),and DAPI (blue).
           引用文献