| 货号: S217094 |
| 产品全名: PSMB7 兔多抗 |
| 基因符号 Z |
| UNIPROT ID: Q99436 (Gene Accession – BC000509 ) |
| 背景: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. The encoded protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. A pseudogene of this gene is located on the long arm of chromosome 14. |
| 抗原: Fusion protein of human PSMB7 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 500-2000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cell Biology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 217094(PSMB7 Antibody) at a dilution of 1/25(Cytoplasm and Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the fusion protein and then with 217094(Anti-PSMB7 Antibody) at dilution 1/25. | 
The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 217094(Anti-PSMB7 Antibody) at a dilution of 1/25. | 
In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with fusion protein and then with D221776(Anti-PSMB7 Antibody) at dilution 1/25. | 
Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: 293T cells, hepG2 cells, human testis tissue, Human lung cancer tissue;
Primary antibody: 217094(PSMB7 Antibody) at dilution 1/310;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds | |
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