| 货号: S219007 |
| 产品全名: PSMB2 兔多抗 |
| 基因符号 HC7-I |
| UNIPROT ID: P49721 (Gene Accession – BC000268 ) |
| 背景: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
| 抗原: Fusion protein of human PSMB2 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 100-300;WB: 1000-5000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cell Biology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 219007(PSMB2 Antibody) at a dilution of 1/130(Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the fusion protein and then with 219007(Anti-PSMB2 Antibody) at dilution 1/130. | 
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 219007(Anti-PSMB2 Antibody) at a dilution of 1/130. | 
In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D225640(Anti-PSMB2 Antibody) at dilution 1/130. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-7: 293T, Jurkat, HT29, K562, LOVO, Hela and HepG2 cell lysates;
Primary antibody: 219007(PSMB2 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds | |
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