| 货号: S218854 |
| 产品全名: PSMA1 兔多抗 |
| 基因符号 NU; HC2; PROS30; HEL-S-275 |
| UNIPROT ID: P25786 (Gene Accession – BC005932 ) |
| 背景: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| 抗原: Fusion protein of human PSMA1 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 150-300;WB: 1000-5000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cell Biology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 218854(PSMA1 Antibody) at a dilution of 1/160(Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the fusion protein and then with 218854(Anti-PSMA1 Antibody) at dilution 1/160. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-7: SKOV3, 293T, Raji, PC-3, HepG2, Hela and Jurkat cell lysates;
Primary antibody: 218854(PSMA1 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 second | |
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