| 货号: S220086 |
| 产品全名: PRKG2 兔多抗 |
| 基因符号 cGKII; PRKGR2 |
| UNIPROT ID: Q13237 (Gene Accession – NP_006250 ) |
| 背景: cGKII (cGMP-dependent protein kinase type II) is a major receptor of intracellular cGMP and mediates a plethora of physiological responses. cGKII contains a conserved leucine zipper motif at the amino-terminus. It is expressed in small intestine, colon, prostate, and human brain tissues, and the cGKII gene maps to chromosome 4q13.1-q21.1. cGKII has been shown to regulate the ion transport system in the intestine. Myristoylation of the penultimate glycine in cGKII appears to be essential for directing cGKII to the membrane, since cGKII is devoid of any hydrophobic transmembrane domains. The translocation of cGKII from the cytosol to the membrane allows it to function properly in regulating intestinal ion transport. |
| 抗原: Synthetic peptide of human PRKG2 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 200-1000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 220086(PRKG2 Antibody) at a dilution of 1/20(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 220086(Anti-PRKG2 Antibody) at dilution 1/20. | 
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 220086(Anti-PRKG2 Antibody) at a dilution of 1/20. | 
In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with synthetic peptide and then with D260929(Anti-PRKG2 Antibody) at dilution 1/20. | 
Gel: 8%SDS-PAGE, Lysate: 50 μg;
Lane 1-2: Mouse brain tissue, Mouse lung tissue;
Primary antibody: 220086(PRKG2 Antibody) at dilution 1/160;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds | |
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