| 货号: S220075 |
| 产品全名: PRKD3 兔多抗 |
| 基因符号 EPK2; PKD3; PRKCN; PKC-NU; nPKC-NU |
| UNIPROT ID: O94806 (Gene Accession – NP_005804 ) |
| 背景: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. |
| 抗原: Synthetic peptide of human PRKD3 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 200-1000;ELISA: 1000-2000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 220075(PRKD3 Antibody) at a dilution of 1/50(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the synthetic peptide and then with 220075(Anti-PRKD3 Antibody) at dilution 1/50. | 
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 220075(Anti-PRKD3 Antibody) at a dilution of 1/50. | 
In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with synthetic peptide and then with D260912(Anti-PRKD3 Antibody) at dilution 1/50. | 
Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-6: Human testis tissue, K562 cells, A549 cells, Raji cells, NIH/3T3 cells, Hela cells;
Primary antibody: 220075(PRKD3 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 minutes | |
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