| 货号: S217482 |
| 产品全名: PPAT 兔多抗 |
| 基因符号 GPAT; PRAT; ATASE |
| UNIPROT ID: Q06203 (Gene Accession – BC004200 ) |
| 背景: The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region. |
| 抗原: Fusion protein of human PPAT |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 100-300;WB: 500-2000;ELISA: 2000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Metabolism, Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 217482(PPAT Antibody) at a dilution of 1/50(Cytoplasm and Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 217482(Anti-PPAT Antibody) at dilution 1/50. | 
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 217482(Anti-PPAT Antibody) at a dilution of 1/50. | 
In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with fusion protein and then with D222426(Anti-PPAT Antibody) at dilution 1/50. | 
Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: Hela cells, Raji cells, Jurkat cells, A549 cells, NIH/3T3 cells;
Primary antibody: 217482(PPAT Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds | |
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