| 货号: N225386 |
| 产品全名: Phospho-Rb (Ser780) 兔多抗 |
| 基因符号 RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110 |
| UNIPROT ID: P06400 |
| 背景: Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo. |
| 抗原: The antiserum was produced against synthesized peptide derived from human Retinoblastoma around the phosphorylation site of Ser780. AA range:751-800 |
| 经过测试的应用: WB,IHC-F,IHC-P,ICC/IF,ELISA |
| 推荐稀释比: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 分子量: Calculated MW: 106 kDa; Observed MW: 106 kDa |
| 亚型: IgG |
| 纯化: Affinity Chromatography |
| 种属反应性: Human, Mouse and Rat |
| Modification: Phosphorylated |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
| 研究领域: Cell Biology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma using Retinoblastoma (Phospho-Ser78) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Sample with blocking peptide on the right. | 
Western blot analysis of Phospho-Rb (Ser780) in various lysates using Phospho-Rb (S780) antibody. | 
Western blot analysis of Phospho-Rb (Ser780) in KB lysates using Phospho-Rb (S780) antibody. | 
Western blot analysis of Phospho-Rb (Ser780) in K562 lysates treated with serum 10% using Phospho-Rb (Ser780) antibody.The lane on the right is blocked with the Phospho- peptide. |
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