| 货号: N262169 |
| 产品全名: Phospho-eIF4E (Ser209) 兔单克隆抗体 |
| 基因符号 EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding 蛋白 |
| UNIPROT ID: P06730 |
| 背景: eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. |
| 抗原: A synthetic phosphopeptide corresponding to residues surrounding Ser209 of human eIF4E |
| 经过测试的应用: WB,IHC-F,IHC-P,ICC/IF,IP |
| 推荐稀释比: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Monoclonal |
| 克隆编号: R04-5A4 |
| 分子量: Calculated MW: 25 kDa; Observed MW: 25 kDa |
| 亚型: IgG |
| 纯化: Affinity Purified |
| 种属反应性: Human, Mouse and Rat |
| Modification: Phosphorylated |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
| 研究领域: Hypoxia Signal Transduction& Hypoxia-induced |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunocytochemistry analysis of eIF4E (Phospho-Ser209)(green) in Hela using eIF4E (Phospho-Ser209) antibody,and DAPI(blue) | 
Western blot analysis of eIF4E (Phospho-Ser209) in K562, rat Brain, C6, 3T3 lysates using Phospho-eIF4E (Ser209) antibody. | 
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Phospho-eIF4E (Ser209) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. | |
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