| 货号: N225415 |
| 产品全名: Phospho-EGFR (Tyr1092) 兔多抗 |
| 基因符号 EGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1 |
| UNIPROT ID: P00533 |
| 背景: EGFR is a receptor tyrosine kinase. Receptor for epidermal growth factor (EGF) and related growth factors including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. . A single-pass transmembrane tyrosine kinase. Ligand binding to this receptor results in receptor dimerization, autophosphorylation (in trans), activation of various downstream signaling molecules and lysosomal degradation. |
| 抗原: The antiserum was produced against synthesized peptide derived from human EGFR around the phosphorylation site of Tyr1092. AA range:1061-1110 |
| 经过测试的应用: WB,IHC-P,ELISA |
| 推荐稀释比: WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 分子量: Calculated MW: 134 kDa; Observed MW: 140-160 kDa |
| 亚型: IgG |
| 纯化: Affinity Chromatography |
| 种属反应性: Human, Mouse and Rat |
| Modification: Phosphorylated |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
| 研究领域: Signal Transduction |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma, using EGFR (Phospho-Tyr109, 2) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. Sample with blocking peptide on the right. | 
Western blot analysis of Phospho-EGFR (Tyr1092) in various lysates using Phospho-EGFR (Tyr1092) antibody. | 
Western blot analysis of Phospho-EGFR (Tyr1092) in HUVEC lysates treated with EGF, using EGFR (Phospho-Tyr19, 2) antibody. The lane on the right is blocked with the Phospho- peptide. | |
|
