| 货号: S218360 |
| 产品全名: PHF21A 兔多抗 |
| 基因符号 BHC80; BM-006 |
| UNIPROT ID: Q96BD5 (Gene Accession – BC015714 ) |
| 背景: Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. PHF21A (PHD finger protein 21A), also known as BRAF35-HDAC complex protein BHC80, is a 680 amino acid nuclear protein that contains one PHD-type zinc finger and one A.T hook DNA-binding domain, suggesting involvement in transcriptional regulation events. PHF21A is a component of the BHC comple,x which is responsible for repressing transcription of neuron-specific genes in non-neuronal cells. The BHC complex acts as a chromatin modifier that deacetylates and demethylates specific sites on histones. PHF21A may act as a scaffold within the BHC complex. Predominantly expressed in brain, three isoforms of PHF21A exist as a result of alternative splicing events. |
| 抗原: Fusion protein of human PHF21A |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 40-200;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling, Neuroscience |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 218360(PHF21A Antibody) at a dilution of 1/50(Cytoplasm and Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the fusion protein and then with 218360(Anti-PHF21A Antibody) at dilution 1/50. | 
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 218360(Anti-PHF21A Antibody) at a dilution of 1/50. | 
In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with fusion protein and then with D224255(Anti-PHF21A Antibody) at dilution 1/50. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-4:A172, 293T, Hela and A375 cell lysates;
Primary antibody: 218360(PHF21A Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 seconds | |
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