| 货号: S213717 |
| 产品全名: PFN2 兔多抗 |
| 基因符号 PFL, D3S1319E |
| UNIPROT ID: P35080 (Gene Accession – NP_444252 ) |
| 背景: The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. There are two alternatively spliced transcript variants encoding different isoforms described for this gene. Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. |
| 抗原: Synthetic peptide of human PFN2 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 500-2000;ELISA: 1000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human brain tissue using 213717(PFN2 Antibody) at a dilution of 1/30(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with the synthetic peptide and then with 213717(Anti-PFN2 Antibody) at dilution 1/30. | 
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 213717(Anti-PFN2 Antibody) at a dilution of 1/30. | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with synthetic peptide and then with D160712(Anti-PFN2 Antibody) at dilution 1/30. | 
Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane: Human fetal brain tissue;
Primary antibody: 213717(PFN2 Antibody) at dilution 1/450;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 minutes | |
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