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PFKFB4 Rabbit pAb

Anti-PFKFB4 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S214142

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S214142
产品全名:   PFKFB4 兔多抗
基因符号   
UNIPROT ID:   Q16877 (Gene Accession – NP_004558 )
背景:   The protein encoded by this gene is one of four bifunctional kinase/phosphatases that regulate the concentration of the glycolytic byproduct fructose-2,6-bisphosphate (F2,6BP). The encoded protein is highly expressed in cancer cells and is induced by hypoxia. This protein is essential to the survival of cancer cells under conditions of hypoxia, because it increases the amount of F2,6BP and ATP at a time when the cell cannot produce much of them. This finding suggests that this protein may be a good target for disruption in cancer cells, hopefully imperiling their survival. Several transcript variants encoding different isoforms have been found for this gene.
抗原:   Synthetic peptide of human PFKFB4
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 25-100;WB: 200-1000;ELISA: 2000-5000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Metabolism, Cancer
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using 214142(PFKFB4 Antibody) at a dilution of 1/35(Cytoplasm).

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Raji, 231, 293T cell lysates;
Primary antibody: 214142(PFKFB4 Antibody) at dilution 1/200;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
           引用文献