武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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PEMT Rabbit pAb

Anti-PEMT Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S216032

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S216032
产品全名:   PEMT 兔多抗
基因符号   PLMT; PNMT; PEAMT; PEMPT; PEMT2
UNIPROT ID:   Q9UBM1 (Gene Accession – NP_009100 )
背景:   Phosphatidylcholine (PC) is the most abundant mammalian phospholipid. This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. Another distinct synthetic pathway in nucleated cells converts intracellular choline to phosphatidylcholine by a three-step process. The protein isoforms encoded by this gene localize to the endoplasmic reticulum and mitochondria-associated membranes. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.
抗原:   Synthetic peptide of human PEMT
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Metabolism, Cancer
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 216032(PEMT Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 216032(Anti-PEMT Antibody) at dilution 1/50.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: Hela, 231, 293T, HepG2 cell lysates;
Primary antibody: 216032(PEMT Antibody) at dilution 1/700;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 10 seconds
           引用文献