| 货号: S219967 |
| 产品全名: PDK4 兔多抗 |
| 基因符号 |
| UNIPROT ID: Q16654 (Gene Accession – NP_002603 ) |
| 背景: This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin. |
| 抗原: Synthetic peptide of human PDK4 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 20-100;WB: 200-1000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Metabolism |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 219967(PDK4 Antibody) at a dilution of 1/20(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the synthetic peptide and then with 219967(Anti-PDK4 Antibody) at dilution 1/20. | 
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 219967(Anti-PDK4 Antibody) at a dilution of 1/20. | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with synthetic peptide and then with D260703(Anti-PDK4 Antibody) at dilution 1/20. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Human placenta tissue lysate;
Primary antibody: 219967(PDK4 Antibody) at dilution 1/400;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 3 minutes | |
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