| 货号: S217605 |
| 产品全名: PCK2 兔多抗 |
| 基因符号 PEPCK; PEPCK2; PEPCK-M |
| UNIPROT ID: Q16822 (Gene Accession – BC001454 ) |
| 背景: This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described. |
| 抗原: Fusion protein of human PCK2 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 100-300;WB: 500-2000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Metabolism, Signal Transduction, Cancer, Cardiovascular |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 217605(PCK2 Antibody) at a dilution of 1/60(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 217605(Anti-PCK2 Antibody) at dilution 1/60. | 
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 217605(Anti-PCK2 Antibody) at a dilution of 1/60. | 
In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with fusion protein and then with D222686(Anti-PCK2 Antibody) at dilution 1/60. | 
Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: 293T cells, Jurkat cells, Hela cells;
Primary antibody: 217605(PCK2 Antibody) at dilution 1/750;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 minutes | |
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