| 货号: S218846 |
| 产品全名: ORC4 兔多抗 |
| 基因符号 ORC4L; ORC4P |
| UNIPROT ID: O43929 (Gene Accession – BC005388 ) |
| 背景: The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. This gene encodes a subunit of the ORC complex. Several alternatively spliced transcript variants, some of which encode the same protein, have been reported for this gene. |
| 抗原: Fusion protein of human ORC4 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-300;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 218846(ORC4 Antibody) at a dilution of 1/80(Nucleus and Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 218846(Anti-ORC4 Antibody) at dilution 1/80. | 
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 218846(Anti-ORC4 Antibody) at a dilution of 1/80. | 
In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D225358(Anti-ORC4 Antibody) at dilution 1/80. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: HepG2 and Hela cell lysates;
Primary antibody: 218846(ORC4 Antibody) at dilution 1/550;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds | |
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