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NRAS Rabbit pAb

Anti-NRAS Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S220763

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S220763
产品全名:   NRAS 兔多抗
基因符号   NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
UNIPROT ID:   P01111 (Gene Accession – NP_002515 )
背景:   This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia.
抗原:   Synthetic peptide of human NRAS
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 50-100;WB: 200-1000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Signal Transduction, Cancer
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 220763(NRAS Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the synthetic peptide and then with 220763(Anti-NRAS Antibody) at dilution 1/50.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Jurkat, 293T cell lysates;
Primary antibody: 220763(NRAS Antibody) at dilution 1/200;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 2 minutes
           引用文献