| 货号: S219944 |
| 产品全名: NPHS1 兔多抗 |
| 基因符号 CNF, NPHN, nephrin |
| UNIPROT ID: O60500 (Gene Accession – NP_004637 ) |
| 背景: This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.In Western blots, nephrin antibodies generated against the two terminal extracellular Ig domains of recombinant human nephrin recognized a 180-kDa protein in lysates of human glomeruli and a 150-kDa protein in transfected COS-7 cell lysates. |
| 抗原: Synthetic peptide of human NPHS1 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 500-2000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human brain tissue using 219944(NPHS1 Antibody) at a dilution of 1/40(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with the synthetic peptide and then with 219944(Anti-NPHS1 Antibody) at dilution 1/40. | 
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 219944(Anti-NPHS1 Antibody) at a dilution of 1/40. | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with synthetic peptide and then with D260661(Anti-NPHS1 Antibody) at dilution 1/40. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Mouse kidney tissue;
Primary antibody: 219944(NPHS1 Antibody) at dilution 1/700;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute | |
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