| 货号: S220148 |
| 产品全名: NOTCH3 兔多抗 |
| 基因符号 IMF2; LMNS; CASIL; CADASIL; CADASIL1 |
| UNIPROT ID: Q9UM47 (Gene Accession – NP_000426 ) |
| 背景: This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). |
| 抗原: Synthetic peptide of human NOTCH3 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 20-100;WB: 200-1000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling, Neuroscience, Cardiovascular, Signal Transduction, Stem Cells |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 220148(NOTCH3 Antibody) at a dilution of 1/20(Cytoplasm or Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the synthetic peptide and then with 220148(Anti-NOTCH3 Antibody) at dilution 1/20. | 
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 220148(Anti-NOTCH3 Antibody) at a dilution of 1/20. | 
In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with synthetic peptide and then with D261043(Anti-NOTCH3 Antibody) at dilution 1/20. | 
Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: MCF7 cell lysate;
Primary antibody: 220148(NOTCH3 Antibody) at dilution 1/400;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute | |
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