| 货号: S220143 |
| 产品全名: NIT2 兔多抗 |
| 基因符号 |
| UNIPROT ID: Q9NQR4 (Gene Accession – NP_064587 ) |
| 背景: Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle. |
| 抗原: Synthetic peptide of human NIT2 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 1000-5000;ELISA: 2000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Metabolism |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 220143(NIT2 Antibody) at a dilution of 1/40(Cytoplasm, Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the synthetic peptide and then with 220143(Anti-NIT2 Antibody) at dilution 1/40. | 
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 220143(Anti-NIT2 Antibody) at a dilution of 1/40. | 
In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with synthetic peptide and then with D261032(Anti-NIT2 Antibody) at dilution 1/40. | 
Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Mouse liver tissue, Mouse kidney tissue, RAW264.7 cells;
Primary antibody: 220143(NIT2 Antibody) at dilution 1/950;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 90 seconds | |
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