| 货号: S219207 |
| 产品全名: MRPL42 兔多抗 |
| 基因符号 L31MT; L42MT; S32MT; MRPL31; MRPS32; PTD007; RPML31; HSPC204; MRP-L31; MRP-L42; MRP-S32 |
| UNIPROT ID: Q9Y6G3 (Gene Accession – BC040240 ) |
| 背景: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein identified as belonging to both the 28S and the 39S subunits. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 4q, 6p, 6q, 7p, and 15q. |
| 抗原: Fusion protein of human MRPL42 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling, Metabolism |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 219207(MRPL42 Antibody) at a dilution of 1/65(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 219207(Anti-MRPL42 Antibody) at dilution 1/65. | 
The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 219207(Anti-MRPL42 Antibody) at a dilution of 1/65. | 
In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with fusion protein and then with D226053(Anti-MRPL42 Antibody) at dilution 1/65. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: Jurkat, HepG2, Hela and Raji cell lysates;
Primary antibody: 219207(MRPL42 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute | |
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