| 货号: S219920 |
| 产品全名: MMP25 兔多抗 |
| 基因符号 MMP20, MMPL1, MMP-25, MMP20A, MT6MMP, MTMMP6, MT-MMP6, MT6-MMP, MT-MMP 6 |
| UNIPROT ID: Q9NPA2 (Gene Accession – NP_071913 ) |
| 背景: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily, attached to the plasma membrane via a glycosylphosphatidyl inositol anchor. In response to bacterial infection or inflammation, the encoded protein is thought to inactivate alpha-1 proteinase inhibitor, a major tissue protectant against proteolytic enzymes released by activated neutrophils, facilitating the transendothelial migration of neutrophils to inflammatory sites. The encoded protein may also play a role in tumor invasion and metastasis through activation of MMP2. The gene has previously been referred to as MMP20 but has been renamed MMP25. |
| 抗原: Synthetic peptide of human MMP25 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 500-2000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction, Cell Biology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 219920(MMP25 Antibody) at a dilution of 1/80(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the synthetic peptide and then with 219920(Anti-MMP25 Antibody) at dilution 1/80. | 
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 219920(Anti-MMP25 Antibody) at a dilution of 1/80. | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with synthetic peptide and then with D260626(Anti-MMP25 Antibody) at dilution 1/80. | 
Gel: 10+12%SDS-PAGE, Lysate: 40 μg;
Lane: 823 cells;
Primary antibody: 219920(MMP25 Antibody) at dilution 1/1300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 minutes | |
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