武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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MGMT Rabbit pAb

Anti-MGMT Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S216645

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S216645
产品全名:   MGMT 兔多抗
基因符号   
UNIPROT ID:   P16455 (Gene Accession – BC000824 )
背景:   Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma.
抗原:   Fusion protein of human MGMT
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Epigenetics and Nuclear Signaling
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 216645(MGMT Antibody) at a dilution of 1/80(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 216645(Anti-MGMT Antibody) at dilution 1/80.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: MCF7, Raji, HT29 cell lysates;
Primary antibody: 216645(MGMT Antibody) at dilution 1/1300;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds
           引用文献