| 货号: S216642 |
| 产品全名: MCM7 兔多抗 |
| 基因符号 MCM2, CDC47, P85MCM, P1CDC47, PNAS146, P1.1-MCM3 |
| UNIPROT ID: P33993 (Gene Accession – BC013375 ) |
| 背景: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. |
| 抗原: Fusion protein of human MCM7 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 15-50;WB: 500-2000;ELISA: 1000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 216642(MCM7 Antibody) at a dilution of 1/15(Cytoplasm, Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the fusion protein and then with 216642(Anti-MCM7 Antibody) at dilution 1/15. | 
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 216642(Anti-MCM7 Antibody) at a dilution of 1/15. | 
In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with fusion protein and then with D220965(Anti-MCM7 Antibody) at dilution 1/15. | 
Gel: 10%SDS-PAGE, Lysate: 50 μg;
Lane: Jurkat cells;
Primary antibody: 216642(MCM7 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds | |
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