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MAPK3 Rabbit pAb

Anti-MAPK3 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S219765

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S219765
产品全名:   MAPK3 兔多抗
基因符号   ERK1, ERT2, ERK-1, PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, p44-ERK1, p44-MAPK
UNIPROT ID:   P27361 (Gene Accession – NP_002737 )
背景:   The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.
抗原:   Synthetic peptide of human MAPK3
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 20-100;WB: 500-2000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Signal Transduction, Cancer, Neuroscience
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 219765(MAPK3 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the synthetic peptide and then with 219765(Anti-MAPK3 Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-7: A375, A549, Hela, HepG2, A431, lovo and 231 cell;
Primary antibody: 219765(MAPK3 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 minutes
           引用文献