| 货号: S221803 |
| 产品全名: MAP1LC3B 兔多抗 |
| 基因符号 LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3 |
| UNIPROT ID: Q9GZQ8 (Gene Accession – NP_073729 ) |
| 背景: The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. |
| 抗原: Synthetic peptide of human MAP1LC3B |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 20-100;WB: 200-1000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction, Cancer, Neuroscience, Cardiovascular |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 221803(MAP1LC3B Antibody) at a dilution of 1/25(Cytoplasm or Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 221803(Anti-MAP1LC3B Antibody) at dilution 1/25. | 
The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using 221803(Anti-MAP1LC3B Antibody) at a dilution of 1/25. | 
In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with synthetic peptide and then with D263557(Anti-MAP1LC3B Antibody) at dilution 1/25. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: HEPG2 cell and Human fetal brain tissue lysates;
Primary antibody: 221803(MAP1LC3B Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds | |
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