| 货号: S216619 |
| 产品全名: LMNA 兔多抗 |
| 基因符号 FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; MADA; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B |
| UNIPROT ID: P02545 (Gene Accession – BC000511 ) |
| 背景: Lamin A/C is also named as LMNA, or LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. The lack of lamin A/C can be as a novel marker for undifferentiated embryonic stem cells and lamin A/C expression is as an early indicator of differentiation. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 74 kDa, 65 kDa, 70 kDa and 64 kDa. This antibody can recognize 4 isoforms of Lamin A/C. |
| 抗原: Fusion protein of human LMNA |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 100-300;WB: 1000-5000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 216619(LMNA Antibody) at a dilution of 1/60(Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the fusion protein and then with 216619(Anti-LMNA Antibody) at dilution 1/60. | 
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 216619(Anti-LMNA Antibody) at a dilution of 1/60. | 
In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with fusion protein and then with D220927(Anti-LMNA Antibody) at dilution 1/60. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: Mouse stomach tissue, HUVEC cell, LOVO cell, Human placenta tissue lysates;
Primary antibody: 216619(LMNA Antibody) at dilution 1/2700;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute | |
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