| 货号: N225340 |
| 产品全名: LIM Kinase 2 兔多抗 |
| 基因符号 LIM domain kinase 2 |
| UNIPROT ID: P53671 |
| 背景: Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics (PubMed:10436159, PubMed:11018042). Acts downstream of several Rho family GTPase signal transduction pathways (PubMed:10436159, PubMed:11018042). Involved in astral microtubule organization and mitotic spindle orientation during early stages of mitosis by mediating phosphorylation of TPPP (PubMed:22328514). Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro (PubMed:8537403). Suppresses ciliogenesis via multiple pathways; phosphorylation of CFL1, suppression of directional trafficking of ciliary vesicles to the ciliary base, and by facilitating YAP1 nuclear localization where it acts as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1 (PubMed:25849865). |
| 抗原: Synthetic peptide of human LIMK2 |
| 经过测试的应用: WB,IHC-P,ICC/IF |
| 推荐稀释比: WB: 1/500-1/1000 IHC: 1/50-1/100 ICC: 1/100-1/200 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 分子量: Calculated MW: 72 kDa; Observed MW: 72 kDa |
| 亚型: IgG |
| 纯化: Affinity Purified |
| 种属反应性: Human, Mouse and Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
| 研究领域: Signal Transduction |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin-embedded Human heart tissue using LIMK2 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. Sample with blocking peptide on the right. | 
Western blot analysis of LIM Kinase 2 in HepG2, HUVEC lysates using LIM Kinase 2 antibody. | 
Immunofluorescence analysis of LIM Kinase 2 in Hela cells using LIMK2 antibody(green). | |
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