| 货号: S222426 |
| 产品全名: LDHB 兔多抗 |
| 基因符号 LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281 |
| UNIPROT ID: P07195 (Gene Accession – NP_002291 ) |
| 背景: This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes ,x 5 and 13. |
| 抗原: Synthetic peptide of human LDHB |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Metabolism, Cancer |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 222426(LDHB Antibody) at a dilution of 1/50(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 222426(Anti-LDHB Antibody) at dilution 1/50. | 
The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 222426(Anti-LDHB Antibody) at a dilution of 1/50. | 
In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with synthetic peptide and then with D264590(Anti-LDHB Antibody) at dilution 1/50. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-7: 293T, Jurkat, A549, Hela, Mouse heart tissue, Mouse kidney tissue, Mouse brain tissue lysates;
Primary antibody: 222426(LDHB Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 5 seconds | |
|
