| 货号: S213626 |
| 产品全名: LAYN 兔多抗 |
| 基因符号 |
| UNIPROT ID: Q6UX15 (Gene Accession – NP_849156 ) |
| 背景: Layilin is a recently cloned ?55-kDa membrane-binding partner for talin (Borowsky and Hynes, 1998 ) that is widely expressed in different cell types and tissue extracts. It is found in peripheral ruffles of spreading cells and is recruited to membrane ruffles in cells induced to migrate in in vitro wounding experiments. Layilin colocalizes with talin in ruffles and binds to talin’s ?50-kDa head domain (amino acids 280?435). Other parts of talin (the ?220-kDa tail fragment) can bind ?-integrin cytoplasmic tails, vinculin, and F-actin and hence form an integrin-cytoskeleton linkage at sites of cell-substratum contact. Layilin’s extracellular domain is homologous with the carbohydrate-recognition domains (CRD) of C-type lectins. Receptor for hyaluronate. |
| 抗原: Synthetic peptide of human LAYN |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-100;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 213626(LAYN Antibody) at a dilution of 1/50(Cell membrane). | 
In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the synthetic peptide and then with 213626(Anti-LAYN Antibody) at dilution 1/50. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Jurkat and K562 cell lysates;
Primary antibody: 213626(LAYN Antibody) at dilution 1/700;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 2 minutes | |
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