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LAT Rabbit pAb

Anti-LAT Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S221459

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S221459
产品全名:   LAT 兔多抗
基因符号   LAT1; pp36
UNIPROT ID:   O43561 (Gene Accession – NP_055202 )
背景:   The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms.
抗原:   Synthetic peptide of human LAT
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 25-100;WB: 200-1000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Signal Transduction, Immunology
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 221459(LAT Antibody) at a dilution of 1/30(Cell membrane and Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the synthetic peptide and then with 221459(Anti-LAT Antibody) at dilution 1/30.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: Human lymph gland tissue lysate;
Primary antibody: 221459(LAT Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
           引用文献