武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

邮箱:2716620741@qq.com

咨询:027 – 63497286 

KRT20 Rabbit pAb

Anti-KRT20 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S221168

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S221168
产品全名:   KRT20 兔多抗
基因符号   K20; CD20; CK20; CK-20; KRT21
UNIPROT ID:   P35900 (Gene Accession – NP_061883 )
背景:   The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21.
抗原:   Synthetic peptide of human KRT20
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 25-100;WB: 500-2000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Signal Transduction, Cancer
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 221168(KRT20 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the synthetic peptide and then with 221168(Anti-KRT20 Antibody) at dilution 1/20.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: LOVO cell and Mouse small intestines tissue lysates;
Primary antibody: 221168(KRT20 Antibody) at dilution 1/600;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 2 minutes
           引用文献