| 货号: S216592 |
| 产品全名: IRF9 兔多抗 |
| 基因符号 p48; IRF-9; ISGF3; ISGF3G |
| UNIPROT ID: Q00978 (Gene Accession – BC035716 ) |
| 背景: Interferon regulatory factor 9 is a protein that in humans is encoded by the IRF9 gene, previously known as ISGF3G. Transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. |
| 抗原: Fusion protein of human IRF9 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 200-1000;ELISA: 1000-2000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling, Immunology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 216592(IRF9 Antibody) at a dilution of 1/50(Cytoplasm or Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 216592(Anti-IRF9 Antibody) at dilution 1/50. | 
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 216592(Anti-IRF9 Antibody) at a dilution of 1/50. | 
In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with fusion protein and then with D220878(Anti-IRF9 Antibody) at dilution 1/50. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Mouse panceas tissue, NIH/3T3 cells;
Primary antibody: 216592(IRF9 Antibody) at dilution 1/380;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1.5 minutes | |
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