Cat.#: S215106 |
Product Name: Anti-IL6 Rabbit Polyclonal Antibody |
Synonyms: CDF; HGF; HSF; BSF2; IL-6; BSF-2; IFNB2; IFN-beta-2 |
UNIPROT ID: P05231 (Gene Accession – NP_000591 ) |
Background: This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). |
Immunogen: Synthetic peptide of human IL6 |
Applications: ELISA, WB, IHC |
Recommended Dilutions: IHC: 50-100;WB: 500-2000;ELISA: 5000-10000 |
Host Species: Rabbit |
Clonality: Rabbit Polyclonal |
Isotype: Immunogen-specific rabbit IgG |
Purification: Antigen affinity purification |
Species Reactivity: Human |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
Research Areas: Immunology, Neuroscience, Microbiology, Cancer, Metabolism |
Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 215106(IL6 Antibody) at a dilution of 1/50(Secreted). | In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the synthetic peptide and then with 215106(Anti-IL6 Antibody) at dilution 1/50. | The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 215106(Anti-IL6 Antibody) at a dilution of 1/50. | In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with synthetic peptide and then with D162892(Anti-IL6 Antibody) at dilution 1/50. | Gel: 12%SDS-PAGE, Lysate: 40 μg; Lane: K562 cell lysate; Primary antibody: 215106(IL6 Antibody) at dilution 1/800; Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution; Exposure time: 1 minute | |
|