| 货号: S216578 |
| 产品全名: IL18 兔多抗 |
| 基因符号 IGIF; IL-18; IL-1g; IL1F4 |
| UNIPROT ID: Q14116 (Gene Accession – BC007007 ) |
| 背景: The protein encoded by this gene is a proinflammatory cytokine of the IL-1 family that is constitutively found as a precursor within the cytoplasm of a variety of cells including macrophages and keratinocytes. The inactive IL-18 precursor is processed to its active form by caspase-1, and is capable of stimulating interferon gamma production, and of regulating both T helper (Th) 1 and Th2 responses. This cytokine has been implicated in the injury of different organs, and in potentially fatal conditions characterized by a cytokine storm. In humans, IL-18 gene is located on chromosome 11. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| 抗原: Fusion protein of human IL18 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-100;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cardiovascular, Cancer, Immunology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 216578(IL18 Antibody) at a dilution of 1/40(Secreted). | 
In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the fusion protein and then with 216578(Anti-IL18 Antibody) at dilution 1/40. | 
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 216578(Anti-IL18 Antibody) at a dilution of 1/40. | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D220831(Anti-IL18 Antibody) at dilution 1/40. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela and HepG2 cell lysates;
Primary antibody: 216578(IL18 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds | |
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