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HPRT1 Rabbit pAb

Anti-HPRT1 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S218842

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S218842
产品全名:   HPRT1 兔多抗
基因符号   HPRT; HGPRT
UNIPROT ID:   P00492 (Gene Accession – BC000578 )
背景:   The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.
抗原:   Fusion protein of human HPRT1
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Epigenetics and Nuclear Signaling, Cell Biology
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 218842(HPRT1 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 218842(Anti-HPRT1 Antibody) at dilution 1/50.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: 293T cell, Mouse brain tissue lysates;
Primary antibody: 218842(HPRT1 Antibody) at dilution 1/700;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 40 seconds
           引用文献