| 货号: S220551 |
| 产品全名: HIF1AN 兔多抗 |
| 基因符号 FIH1 |
| UNIPROT ID: Q9NWT6 (Gene Accession – NP_060372 ) |
| 背景: Hypoxia-inducible factor 1-alpha inhibitor is a protein that in humans is encoded by the HIF1AN gene. Hydroxylates HIF-1 alpha at ‘Asp-803’ in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins |
| 抗原: Synthetic peptide of human HIF1AN |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 500-2000;ELISA: 1000-2000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cardiovascular, Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human brain tissue using 220551(HIF1AN Antibody) at a dilution of 1/25(Nucleus and Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with the synthetic peptide and then with 220551(Anti-HIF1AN Antibody) at dilution 1/25. | 
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 220551(Anti-HIF1AN Antibody) at a dilution of 1/25. | 
In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with synthetic peptide and then with D261682(Anti-HIF1AN Antibody) at dilution 1/25. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: 231 cells;
Primary antibody: 220551(HIF1AN Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds | |
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