| 货号: N225447 |
| 产品全名: HDAC5 兔多抗 |
| 基因符号 HD5; NY-CO-9 |
| UNIPROT ID: Q9UQL6 |
| 背景: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. |
| 抗原: The antiserum was produced against synthesized peptide derived from human HDAC5. AA range:464-513 |
| 经过测试的应用: WB,IHC-F,IHC-P,ICC/IF,ELISA |
| 推荐稀释比: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 分子量: Calculated MW: 122 kDa; Observed MW: 122 kDa |
| 亚型: IgG |
| 纯化: Affinity Chromatography |
| 种属反应性: Human,Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue using HDAC5 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. Sample with blocking peptide on the right. | 
Western blot analysis of HDAC5 in various lysates using HDAC5 antibody. | 
Western blot analysis of HDAC5 in K562 lysates using HDAC5 antibody. | 
Western blot analysis of HDAC5 in colo lysates using HDAC5 antibody.The lane on the right is blocked with the synthesized peptide. |
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