| 货号: S219849 |
| 产品全名: HDAC1 兔多抗 |
| 基因符号 HD1, RPD3, GON-10, RPD3L1 |
| UNIPROT ID: Q13547 (Gene Accession – NP_004955 ) |
| 背景: Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. |
| 抗原: Synthetic peptide of human HDAC1 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 200-1000;ELISA: 1000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling, Cardiovascular, Signal Transduction |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 219849(HDAC1 Antibody) at a dilution of 1/20(Cytoplasm, Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the synthetic peptide and then with 219849(Anti-HDAC1 Antibody) at dilution 1/20. | 
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 219849(Anti-HDAC1 Antibody) at a dilution of 1/20. | 
In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with synthetic peptide and then with D260479(Anti-HDAC1 Antibody) at dilution 1/20. | 
Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Human colon tissue, Human kidney cancer tissue;
Primary antibody: 219849(HDAC1 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 minutes | |
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