| 货号: S220599 |
| 产品全名: H2AC4 兔多抗 |
| 基因符号 H2A/m; H2AFM; HIST1H2AB |
| UNIPROT ID: P04908 (Gene Accession – NP_003504 ) |
| 背景: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| 抗原: Synthetic peptide of human H2AC4 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 200-1000;ELISA: 1000-2000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 220599(H2AC4 Antibody) at a dilution of 1/30(Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the synthetic peptide and then with 220599(Anti-H2AC4 Antibody) at dilution 1/30. | 
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 220599(Anti-H2AC4 Antibody) at a dilution of 1/30. | 
In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with synthetic peptide and then with D261744(Anti-H2AC4 Antibody) at dilution 1/30. | 
Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: Hela cells, 231 cells, K562 cells, human fetal brain tissue;
Primary antibody: 220599(H2AC4 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 minutes | |
|
