| 货号: S222152 |
| 产品全名: GTF3A 兔多抗 |
| 基因符号 AP2; TFIIIA |
| UNIPROT ID: Q92664 (Gene Accession – NP_002088 ) |
| 背景: The product of this gene is a zinc finger protein with nine Cis[2]-His[2] zinc finger domains. It functions as an RNA polymerase III transcription factor to induce transcription of the 5S rRNA genes. The protein binds to a 50 bp internal promoter in the 5S genes called the internal control region (ICR), and nucleates formation of a stable preinitiation complex. This complex recruits the TFIIIC and TFIIIB transcription factors and RNA polymerase III to form the complete transcription complex. The protein is thought to be translated using a non-AUG translation initiation site in mammals based on sequence analysis, protein homology, and the size of the purified protein. |
| 抗原: Synthetic peptide of human GTF3A |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-300;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 222152(GTF3A Antibody) at a dilution of 1/60(Nucleus and Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the synthetic peptide and then with 222152(Anti-GTF3A Antibody) at dilution 1/60. | 
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 222152(Anti-GTF3A Antibody) at a dilution of 1/60. | 
In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with synthetic peptide and then with D264139(Anti-GTF3A Antibody) at dilution 1/60. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: TM4 cell lysate;
Primary antibody: 222152(GTF3A Antibody) at dilution 1/550;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 7 seconds | |
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