Cat.#: S220577 |
Product Name: Anti-GLS Rabbit Polyclonal Antibody |
Synonyms: GAC; GAM; KGA; GLS1; AAD20 |
UNIPROT ID: O94925 (Gene Accession – NP_001243239 ) |
Background: This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. |
Immunogen: Synthetic peptide of human GLS |
Applications: ELISA, WB, IHC |
Recommended Dilutions: IHC: 50-100;WB: 500-2000;ELISA: 5000-10000 |
Host Species: Rabbit |
Clonality: Rabbit Polyclonal |
Isotype: Immunogen-specific rabbit IgG |
Purification: Antigen affinity purification |
Species Reactivity: Human, Mouse, Rat |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
Research Areas: Metabolism, Neuroscience |
Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 220577(GLS Antibody) at a dilution of 1/40(Cytoplasm). | In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 220577(Anti-GLS Antibody) at dilution 1/40. | Gel: 8%SDS-PAGE, Lysate: 40 μg; Lane 1-2: Hela and HT-29 cell lysates; Primary antibody: 220577(GLS Antibody) at dilution 1/650; Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution; Exposure time: 10 seconds | |
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