| 货号: S210014 |
| 产品全名: GAPDH 兔多抗 |
| 基因符号 G3PD; GAPD; HEL-S-162eP |
| UNIPROT ID: P04406 (Gene Accession – BC001601 ) |
| 背景: This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. |
| 抗原: Fusion protein of human GAPDH |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 5000-10000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Metabolism, Cancer, Neuroscience |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 210014(GAPDH Antibody) at a dilution of 1/70(Cytoplasm and Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 210014(Anti-GAPDH Antibody) at dilution 1/70. | 
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 210014(Anti-GAPDH Antibody) at a dilution of 1/70. | 
In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with fusion protein and then with D110016(Anti-GAPDH Antibody) at dilution 1/70. | 
Gel: 8%SDS-PAGE, Lysate:40 μg;
Lane 1-5: Raji, HepG2, K562, PC-3, HT-29 cell lysates;
Primary antibody: 210014(GAPDH Antibody) at dilution 1/4000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 second | |
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