| 货号: S216265 |
| 产品全名: FAS 兔多抗 |
| 基因符号 APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6 |
| UNIPROT ID: P25445 (Gene Accession – BC012479 ) |
| 背景: The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. |
| 抗原: Fusion protein of human FAS |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 250-500;WB: 1000-5000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cancer, Immunology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 216265(FAS Antibody) at a dilution of 1/280(Secreted). | 
In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the fusion protein and then with 216265(Anti-FAS Antibody) at dilution 1/280. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Raji cell lysate;
Primary antibody: 216265(FAS Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 second | |
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