| 货号: S217549 |
| 产品全名: F11R 兔多抗 |
| 基因符号 JAM; KAT; JAM1; JAMA; JCAM; CD321; PAM-1 |
| UNIPROT ID: Q9Y624 (Gene Accession – BC001533 ) |
| 背景: Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as a receptor for reovirus, a ligand for the integrin LFA1, involved in leukocyte transmigration, and a platelet receptor. Multiple 5′ alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established. |
| 抗原: Fusion protein of human F11R |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 500-2000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction, Cardiovascular |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 217549(F11R Antibody) at a dilution of 1/60(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 217549(Anti-F11R Antibody) at dilution 1/60. | 
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 217549(Anti-F11R Antibody) at a dilution of 1/60. | 
In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with fusion protein and then with D222560(Anti-F11R Antibody) at dilution 1/60. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: K562 cells, human kidney cancer tissue, 293T cells, HepG2 cells;
Primary antibody: 217549(F11R Antibody) at dilution 1/600;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds | |
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