| 货号: S218690 |
| 产品全名: EIF4A3 兔多抗 |
| 基因符号 RCPS; DDX48; MUK34; NUK34; NMP265; eIF4AIII |
| UNIPROT ID: P38919 (Gene Accession – BC003662 ) |
| 背景: This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family. |
| 抗原: Fusion protein of human EIF4A3 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-300;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 218690(EIF4A3 Antibody) at a dilution of 1/45(Nucleus and Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 218690(Anti-EIF4A3 Antibody) at dilution 1/45. | 
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 218690(Anti-EIF4A3 Antibody) at a dilution of 1/45. | 
In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D224949(Anti-EIF4A3 Antibody) at dilution 1/45. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: Raji, Hela, A549, HEPG2 and 231 cell lysates;
Primary antibody: 218690(EIF4A3 Antibody) at dilution 1/500;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 15 seconds | |
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